Journal: Critical Care

Article Title: Combination of serum and peritoneal 1.3-beta- d -glucan can rule out intra-abdominal candidiasis in surgical critically ill patients: a multicenter prospective study

doi: 10.1186/s13054-023-04761-7

Figure Lengend Snippet: Study design and inclusion criteria. Abbreviations: BDG: 1.3 beta- d -glucan; IAC: intra-abdominal infection; IAI: intra-abdominal infection. Risk factors according to [ – ], Peritonitis score according to

Article Snippet: Wako ® beta-glucan test (WT, Fujifilm Wako Chemicals Europe, Neuss, Germany) was used for pBDG measurements.

Techniques: Infection

Journal: Critical Care

Article Title: Combination of serum and peritoneal 1.3-beta- d -glucan can rule out intra-abdominal candidiasis in surgical critically ill patients: a multicenter prospective study

doi: 10.1186/s13054-023-04761-7

Figure Lengend Snippet: Serum and peritoneal 1.3 beta- d -glucan concentrations in IAC patients versus non-IAC. A Box and whiskers with median, 10 and 90% percentile of peritoneal BDG between confirmed IAC and non-IAC patients (Wako ® beta-glucan test, Fujifilm Wako Chemicals Europe, Neuss, Germany). Dotted line represents the threshold of 45 pg/ml. B Box and whiskers with median, 10 and 90% percentile of serum BDG measured with the Wako ® beta-glucan test (Fujifilm Wako Chemicals Europe, Neuss, Germany) at Day 1 between confirmed IAC and non-IAC patients. Dotted line represents the threshold of 3.3 pg/ml (WT). C Box and whiskers with median, 10 and 90% percentile of serum BDG measured with the Fungitell ® beta- d -glucan assay (FA, Associate of Cape Cod, East Falmouth, Inc., United States of America) at Day 1 between confirmed IAC and non-IAC patients. Dotted line represents the threshold of 80 pg/ml (FA). Abbreviations: IAC: intra-abdominal candidiasis; BDG: 1.3 beta- d -glucan; WT: Wako ® beta-glucan test (Fujifilm Wako Chemicals Europe, Neuss, Germany); FA: Fungitell ® beta- d -glucan assay (Associate of Cape Cod, East Falmouth, Inc., United States of America)

Article Snippet: Wako ® beta-glucan test (WT, Fujifilm Wako Chemicals Europe, Neuss, Germany) was used for pBDG measurements.

Techniques:

Journal: Critical Care

Article Title: Combination of serum and peritoneal 1.3-beta- d -glucan can rule out intra-abdominal candidiasis in surgical critically ill patients: a multicenter prospective study

doi: 10.1186/s13054-023-04761-7

Figure Lengend Snippet: Peritoneal and serum 1.3 beta- d -glucan to rule out intra-abdominal candidiasis in secondary peritonitis. Receiver operating characteristic curve of peritoneal and serum 1.3 beta- d -glucan for identification of intra-abdominal candidiasis ( A —peritoneal BDG (n = 196)/ B —serum BDG at Day 1 according to the test used (Wako test ® N = 42 and Fungitell ® beta- d -glucan assay N = 140). Abbreviations: FA: Fungitell ® beta- d -glucan assay (Associate of Cape Cod, East Falmouth, Inc., United States of America); pBDG: peritoneal 1.3 beta- d -glucan; sBDG: serum 1.3 beta- d -glucan; WT: Wako ® beta-glucan test (Fujifilm Wako Chemicals Europe, Neuss, Germany)

Article Snippet: Wako ® beta-glucan test (WT, Fujifilm Wako Chemicals Europe, Neuss, Germany) was used for pBDG measurements.

Techniques:

Journal: Antibiotics

Article Title: Screening Methods for Antimicrobial Residues in the Dairy Chain—The Past and the Present

doi: 10.3390/antibiotics13111098

Figure Lengend Snippet: Examples of rapid specific tests for the detection of antimicrobial residues in milk.

Article Snippet: Beta-lactams+ Tetracyclines BT Combo Test Kit , Ring Biotechnology Co., Ltd., Beijing, China , beta-lactams, tetracyclines , Visually.

Techniques:

Journal: Planta medica

Article Title: Total bacterial load within Echinacea purpurea , determined using a new PCR-based quantification method, is correlated with LPS levels and in vitro macrophage activity

doi: 10.1055/s-0032-1328023

Figure Lengend Snippet: Correlations between bacterial load, THP-1 monocyte stimulatory activity and LPS levels in extracts from Echinacea. E. purpurea root and herb (aerial) plant parts were obtained as bulk raw material from six commercial growers (11 samples) and an individual plant cultivated at the University of Mississippi (herb and root). Plant material was extracted with 4% SDS and crude extracts assessed for activity in THP-1 monocytes transfected with a NF-κB luciferase reporter plasmid (EC50 values of extracts are expressed in μg of plant material/mL, ranging between 3 and 1,940). The LPS content of each extract, expressed as EU/g of dried plant material (levels ranged between 10 and 121,160), was determined using the Chromo-LAL assay with Glucashield® (1→3)-β-D-Glucan Inhibiting Buffer. Total bacterial load in dried plant samples was estimated as described in Fig. 1. Relationships are shown as pairwise linear regressions between macrophage stimulatory extract activity and total bacterial load of plant material (a), extract LPS levels and total bacterial load of plant material (b), and macrophage stimulatory activity and LPS levels in plant extracts (c). Regression analyses were performed on log10 transformed data using Microsoft Excel 2007.

Article Snippet: Amount of LPS (bacterial endotoxin) in extracts was determined using a LAL assay (Chromo-LAL test kit with Glucashield ® (1→3)-β-D-Glucan Inhibiting Buffer) from Associates of Cape Cod Inc. (East Falmouth, MA).

Techniques: Activity Assay, Raw Material, Transfection, Luciferase, Plasmid Preparation, LAL Assay, Transformation Assay